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KMID : 0613820180280090999
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Journal of Life Science
2018 Volume.28 No. 9 p.999 ~ p.1006
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Artemisia scoparia Inhibits Adipogenesis in 3T3-L1 Pre-adipocytes by Downregulating the MAPK Pathway
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Oh Jung-Hwan
Fatih Karadeniz
Seo Young-Wan
Kong Chang-Suk
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Abstract
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Obesity is epidemic worldwide and has reportedly been linked to the progression of several metabolic and cardiovascular diseases. The natural products are decreasing the side effects of medicines used for obesity and also have health benefits dut to their numerous bioactive compounds. In this context, Artemisia scoparia is a widespread plant that has been suggested as possessing various types of bioactivity. In this study, the crude extract from A. scoparia (ASE) was tested for its ability to suppress adipogenesis in mouse 3T3-L1 pre-adipocytes. The molecular pathway by which ASE affects differentiation of 3T3-L1 cells was also investigated. The introduction of ASE to differentiating 3T3-L1 pre-adipocytes resulted in suppressed adipogenesis, as confirmed by decreased intracellular lipid accumulation. The differentiating cells treated with 10 and 100 μg/ml of ASE showed 21.9 and 29.0% less lipid accumulation, respectively, than untreated adipocytes. In addition, the results indicated that ASE treatment lowered the expression of the adipogenesis-related factors PPARγ, C/EBPα, and SREBP-1c. Furthermore, treating with ASE notably decreased levels of phosphorylated p38, ERK, and JNK in 3T3-L1 adipocytes. These results indicate that ASE exhibits significant anti-adipogenesis activity by downregulating the MAPK and PPARγ pathways during the differentiation of 3T3-L1 pre- adipocytes. Therefore, A. scoparia may be a potential source of natural products against obesity.
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KEYWORD
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Adipogenesis, Artemisia scoparia, MAPK, PPARγ, 3T3-L1
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